Electromagnetic field investigation on different cancer cell lines

2014
Authors
Filipović, Nenad
Đukić, Tijana

Radović, Miloš
Cvetković, Danijela
Ćurčić, Milena

Marković, Snežana

Peulić, Aleksandar

Jeremić, Branislav
Article (Published version)
Metadata
Show full item recordAbstract
Background: There is a strong interest in the investigation of extremely low frequency Electromagnetic Fields (EMF) in the clinic. While evidence about anticancer effects exists, the mechanism explaining this effect is still unknown. Methods: We investigated in vitro, and with computer simulation, the influence of a 50 Hz EMF on three cancer cell lines: breast cancer MDA-MB-231, and colon cancer SW-480 and HCT-116. After 24 h preincubation, cells were exposed to 50 Hz extremely low frequency (ELF) radiofrequency EMF using in vitro exposure systems for 24 and 72 h. A computer reaction-diffusion model with the net rate of cell proliferation and effect of EMF in time was developed. The fitting procedure for estimation of the computer model parameters was implemented. Results: Experimental results clearly showed disintegration of cells treated with a 50 Hz EMF, compared to untreated control cells. A large percentage of treated cells resulted in increased early apoptosis after 24 h and 72 h..., compared to the controls. Computer model have shown good comparison with experimental data. Conclusion: Using EMF at specific frequencies may represent a new approach in controlling the growth of cancer cells, while computer modelling could be used to predict such effects and make optimisation for complex experimental design. Further studies are required before testing this approach in humans.
Keywords:
Electromagnetic fields / Computer simulation / Breast cancer / Colon cancerSource:
Cancer Cell International, 2014, 14Publisher:
- BMC, London
Funding / projects:
- Application of biomedical engineering for preclinical and clinical practice (RS-41007)
- Preclinical investigation of bioactive substances (RS-41010)
DOI: 10.1186/s12935-014-0084-x
ISSN: 1475-2867
WoS: 000340965300001
Scopus: 2-s2.0-84906829363
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Geografski fakultetTY - JOUR AU - Filipović, Nenad AU - Đukić, Tijana AU - Radović, Miloš AU - Cvetković, Danijela AU - Ćurčić, Milena AU - Marković, Snežana AU - Peulić, Aleksandar AU - Jeremić, Branislav PY - 2014 UR - https://gery.gef.bg.ac.rs/handle/123456789/605 AB - Background: There is a strong interest in the investigation of extremely low frequency Electromagnetic Fields (EMF) in the clinic. While evidence about anticancer effects exists, the mechanism explaining this effect is still unknown. Methods: We investigated in vitro, and with computer simulation, the influence of a 50 Hz EMF on three cancer cell lines: breast cancer MDA-MB-231, and colon cancer SW-480 and HCT-116. After 24 h preincubation, cells were exposed to 50 Hz extremely low frequency (ELF) radiofrequency EMF using in vitro exposure systems for 24 and 72 h. A computer reaction-diffusion model with the net rate of cell proliferation and effect of EMF in time was developed. The fitting procedure for estimation of the computer model parameters was implemented. Results: Experimental results clearly showed disintegration of cells treated with a 50 Hz EMF, compared to untreated control cells. A large percentage of treated cells resulted in increased early apoptosis after 24 h and 72 h, compared to the controls. Computer model have shown good comparison with experimental data. Conclusion: Using EMF at specific frequencies may represent a new approach in controlling the growth of cancer cells, while computer modelling could be used to predict such effects and make optimisation for complex experimental design. Further studies are required before testing this approach in humans. PB - BMC, London T2 - Cancer Cell International T1 - Electromagnetic field investigation on different cancer cell lines VL - 14 DO - 10.1186/s12935-014-0084-x UR - https://hdl.handle.net/21.15107/rcub_gery_605 ER -
@article{ author = "Filipović, Nenad and Đukić, Tijana and Radović, Miloš and Cvetković, Danijela and Ćurčić, Milena and Marković, Snežana and Peulić, Aleksandar and Jeremić, Branislav", year = "2014", abstract = "Background: There is a strong interest in the investigation of extremely low frequency Electromagnetic Fields (EMF) in the clinic. While evidence about anticancer effects exists, the mechanism explaining this effect is still unknown. Methods: We investigated in vitro, and with computer simulation, the influence of a 50 Hz EMF on three cancer cell lines: breast cancer MDA-MB-231, and colon cancer SW-480 and HCT-116. After 24 h preincubation, cells were exposed to 50 Hz extremely low frequency (ELF) radiofrequency EMF using in vitro exposure systems for 24 and 72 h. A computer reaction-diffusion model with the net rate of cell proliferation and effect of EMF in time was developed. The fitting procedure for estimation of the computer model parameters was implemented. Results: Experimental results clearly showed disintegration of cells treated with a 50 Hz EMF, compared to untreated control cells. A large percentage of treated cells resulted in increased early apoptosis after 24 h and 72 h, compared to the controls. Computer model have shown good comparison with experimental data. Conclusion: Using EMF at specific frequencies may represent a new approach in controlling the growth of cancer cells, while computer modelling could be used to predict such effects and make optimisation for complex experimental design. Further studies are required before testing this approach in humans.", publisher = "BMC, London", journal = "Cancer Cell International", title = "Electromagnetic field investigation on different cancer cell lines", volume = "14", doi = "10.1186/s12935-014-0084-x", url = "https://hdl.handle.net/21.15107/rcub_gery_605" }
Filipović, N., Đukić, T., Radović, M., Cvetković, D., Ćurčić, M., Marković, S., Peulić, A.,& Jeremić, B.. (2014). Electromagnetic field investigation on different cancer cell lines. in Cancer Cell International BMC, London., 14. https://doi.org/10.1186/s12935-014-0084-x https://hdl.handle.net/21.15107/rcub_gery_605
Filipović N, Đukić T, Radović M, Cvetković D, Ćurčić M, Marković S, Peulić A, Jeremić B. Electromagnetic field investigation on different cancer cell lines. in Cancer Cell International. 2014;14. doi:10.1186/s12935-014-0084-x https://hdl.handle.net/21.15107/rcub_gery_605 .
Filipović, Nenad, Đukić, Tijana, Radović, Miloš, Cvetković, Danijela, Ćurčić, Milena, Marković, Snežana, Peulić, Aleksandar, Jeremić, Branislav, "Electromagnetic field investigation on different cancer cell lines" in Cancer Cell International, 14 (2014), https://doi.org/10.1186/s12935-014-0084-x ., https://hdl.handle.net/21.15107/rcub_gery_605 .