Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers
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Autori
Lučić, AleksandarPopović, Vladan
Nevenić, Marija
Ristić, Danijela
Rakonjac, Ljubinko
Ćirković-Mitrović, Tatjana
Mladenović-Drinić, Snežana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpret...ing genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).
Ključne reči:
Scots pine / SSR / UPGMA / correspondence analysisIzvor:
Archives of Biological Sciences, 2014, 66, 4, 1485-1492Izdavač:
- Belgrade : Serbian Biological Society
Finansiranje / projekti:
- Razvoj tehnoloških postupaka u šumarstvu u cilju realizacije optimalne šumovitosti (RS-MESTD-Technological Development (TD or TR)-31070)
DOI: 10.2298/ABS1404485L
ISSN: 0354-4664
WoS: 000346477900023
Scopus: 2-s2.0-84921646941
Kolekcije
Institucija/grupa
Geografski fakultetTY - JOUR AU - Lučić, Aleksandar AU - Popović, Vladan AU - Nevenić, Marija AU - Ristić, Danijela AU - Rakonjac, Ljubinko AU - Ćirković-Mitrović, Tatjana AU - Mladenović-Drinić, Snežana PY - 2014 UR - http://gery.gef.bg.ac.rs/handle/123456789/1547 AB - This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis). PB - Belgrade : Serbian Biological Society T2 - Archives of Biological Sciences T1 - Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers VL - 66 IS - 4 SP - 1485 EP - 1492 DO - 10.2298/ABS1404485L ER -
@article{ author = "Lučić, Aleksandar and Popović, Vladan and Nevenić, Marija and Ristić, Danijela and Rakonjac, Ljubinko and Ćirković-Mitrović, Tatjana and Mladenović-Drinić, Snežana", year = "2014", abstract = "This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).", publisher = "Belgrade : Serbian Biological Society", journal = "Archives of Biological Sciences", title = "Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers", volume = "66", number = "4", pages = "1485-1492", doi = "10.2298/ABS1404485L" }
Lučić, A., Popović, V., Nevenić, M., Ristić, D., Rakonjac, L., Ćirković-Mitrović, T.,& Mladenović-Drinić, S.. (2014). Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers. in Archives of Biological Sciences Belgrade : Serbian Biological Society., 66(4), 1485-1492. https://doi.org/10.2298/ABS1404485L
Lučić A, Popović V, Nevenić M, Ristić D, Rakonjac L, Ćirković-Mitrović T, Mladenović-Drinić S. Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers. in Archives of Biological Sciences. 2014;66(4):1485-1492. doi:10.2298/ABS1404485L .
Lučić, Aleksandar, Popović, Vladan, Nevenić, Marija, Ristić, Danijela, Rakonjac, Ljubinko, Ćirković-Mitrović, Tatjana, Mladenović-Drinić, Snežana, "Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers" in Archives of Biological Sciences, 66, no. 4 (2014):1485-1492, https://doi.org/10.2298/ABS1404485L . .