TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša
AU  - Ćurčić, Nina
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1396
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media SA
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša and Ćurčić, Nina and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media SA",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S., Ćurčić, N., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution
Frontiers Media SA., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković S, Ćurčić N, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša, Ćurčić, Nina, Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities" in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Marjanović, Miloš
AU  - Milenković, Jelena
AU  - Lukić, Milica
AU  - Tomić, Nemanja
AU  - Antić, Aleksandar
AU  - Marković, Rastko S
AU  - Atanasijević, Jasna
AU  - Božić, Dragana
AU  - Buhmiler, Sandra
AU  - Radaković, Milica
AU  - Radivojević, Aleksandar R
AU  - Langović Milićević, Ana
AU  - Gavrilov, Milivoj B
AU  - Marković, Slobodan B
PY  - 2022
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1348
AB  - Mt. Stara Planina is located in the eastern part of the Republic of Serbia and represents the westernmost part of the large mountain massif of the Balkans. Both endogenous and exogenous forces created interesting geomorphological and hydrological features for geotourism development in this area. This article proposes a preliminary list of geomorphological and hydrological sites and analyses them to reveal which geosite possesses geotourism potential. This research was carried out by applying the modified geosite assessment model. In this article, ten geosites were singled out based on the degree of their attractiveness for geotourism development. The results reveal information about the key fields of improvement for each evaluated geosite, and identify which areas require more attention and better management in the upcoming period for Mt. Stara Planina to become a well-known geotourism destination that would attract a large number of tourists in the future.
PB  - De Gruyter Open Access
T2  - Open Geosciences
T1  - Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination
VL  - 14
IS  - 1
SP  - 275
EP  - 293
DO  - 10.1515/geo-2022-0340
ER  - 

@article{
author = "Marjanović, Miloš and Milenković, Jelena and Lukić, Milica and Tomić, Nemanja and Antić, Aleksandar and Marković, Rastko S and Atanasijević, Jasna and Božić, Dragana and Buhmiler, Sandra and Radaković, Milica and Radivojević, Aleksandar R and Langović Milićević, Ana and Gavrilov, Milivoj B and Marković, Slobodan B",
year = "2022",
abstract = "Mt. Stara Planina is located in the eastern part of the Republic of Serbia and represents the westernmost part of the large mountain massif of the Balkans. Both endogenous and exogenous forces created interesting geomorphological and hydrological features for geotourism development in this area. This article proposes a preliminary list of geomorphological and hydrological sites and analyses them to reveal which geosite possesses geotourism potential. This research was carried out by applying the modified geosite assessment model. In this article, ten geosites were singled out based on the degree of their attractiveness for geotourism development. The results reveal information about the key fields of improvement for each evaluated geosite, and identify which areas require more attention and better management in the upcoming period for Mt. Stara Planina to become a well-known geotourism destination that would attract a large number of tourists in the future.",
publisher = "De Gruyter Open Access",
journal = "Open Geosciences",
title = "Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination",
volume = "14",
number = "1",
pages = "275-293",
doi = "10.1515/geo-2022-0340"
}

Marjanović, M., Milenković, J., Lukić, M., Tomić, N., Antić, A., Marković, R. S., Atanasijević, J., Božić, D., Buhmiler, S., Radaković, M., Radivojević, A. R., Langović Milićević, A., Gavrilov, M. B.,& Marković, S. B.. (2022). Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination. in Open Geosciences
De Gruyter Open Access., 14(1), 275-293.
https://doi.org/10.1515/geo-2022-0340

Marjanović M, Milenković J, Lukić M, Tomić N, Antić A, Marković RS, Atanasijević J, Božić D, Buhmiler S, Radaković M, Radivojević AR, Langović Milićević A, Gavrilov MB, Marković SB. Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination. in Open Geosciences. 2022;14(1):275-293.
doi:10.1515/geo-2022-0340 .

Marjanović, Miloš, Milenković, Jelena, Lukić, Milica, Tomić, Nemanja, Antić, Aleksandar, Marković, Rastko S, Atanasijević, Jasna, Božić, Dragana, Buhmiler, Sandra, Radaković, Milica, Radivojević, Aleksandar R, Langović Milićević, Ana, Gavrilov, Milivoj B, Marković, Slobodan B, "Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination" in Open Geosciences, 14, no. 1 (2022):275-293,
https://doi.org/10.1515/geo-2022-0340 . .

TY  - JOUR
AU  - Stričević, Ljiljana S.
AU  - Pavlović, Mila
AU  - Filipović, Ivan M.
AU  - Radivojević, Aleksandar
AU  - Martić-Bursać, Nataša
AU  - Gocić, Milena
PY  - 2021
UR  - https://gery.gef.bg.ac.rs/handle/123456789/1080
AB  - This paper analyzes water quality from four hydrological stations in the Nisava river basin from 2009-2018 based on the following parameters: pH, conductivity, O-2 saturation, BOD5, suspended solids, total oxidized nitrogen, phosphates, turbidity and coliform bacteria. Authors have applied WQI (water quality index) as the most reliable indicator of the watercourses pollution for setting of surface water flow quality. Statistical analysis of variance (ANOVA) using t-test inferential statistical analysis and post-hoc Tukey test were applied to evaluate statistically significant differences between specific data groups. The results show that bad water quality was registered on all the profiles on an annual basis (WQI = 65-71). The river water pH decreases downstream, while values of BOD5, suspended solids, turbidity, TON, phosphates and coliform bacteria gradually increase. The waters of the Nisava at the station Nis belong to the third class of waters and they are mostly loaded with organic matter originating from waste sewage and industrial waters of both urban and rural areas.
PB  - Czech Geographic Soc, Prague 2
T2  - Geografie
T1  - Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018
VL  - 126
IS  - 1
SP  - 55
EP  - 73
DO  - 10.37040/geografie2021126010055
UR  - https://hdl.handle.net/21.15107/rcub_gery_1080
ER  - 

@article{
author = "Stričević, Ljiljana S. and Pavlović, Mila and Filipović, Ivan M. and Radivojević, Aleksandar and Martić-Bursać, Nataša and Gocić, Milena",
year = "2021",
abstract = "This paper analyzes water quality from four hydrological stations in the Nisava river basin from 2009-2018 based on the following parameters: pH, conductivity, O-2 saturation, BOD5, suspended solids, total oxidized nitrogen, phosphates, turbidity and coliform bacteria. Authors have applied WQI (water quality index) as the most reliable indicator of the watercourses pollution for setting of surface water flow quality. Statistical analysis of variance (ANOVA) using t-test inferential statistical analysis and post-hoc Tukey test were applied to evaluate statistically significant differences between specific data groups. The results show that bad water quality was registered on all the profiles on an annual basis (WQI = 65-71). The river water pH decreases downstream, while values of BOD5, suspended solids, turbidity, TON, phosphates and coliform bacteria gradually increase. The waters of the Nisava at the station Nis belong to the third class of waters and they are mostly loaded with organic matter originating from waste sewage and industrial waters of both urban and rural areas.",
publisher = "Czech Geographic Soc, Prague 2",
journal = "Geografie",
title = "Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018",
volume = "126",
number = "1",
pages = "55-73",
doi = "10.37040/geografie2021126010055",
url = "https://hdl.handle.net/21.15107/rcub_gery_1080"
}

Stričević, L. S., Pavlović, M., Filipović, I. M., Radivojević, A., Martić-Bursać, N.,& Gocić, M.. (2021). Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018. in Geografie
Czech Geographic Soc, Prague 2., 126(1), 55-73.
https://doi.org/10.37040/geografie2021126010055
https://hdl.handle.net/21.15107/rcub_gery_1080

Stričević LS, Pavlović M, Filipović IM, Radivojević A, Martić-Bursać N, Gocić M. Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018. in Geografie. 2021;126(1):55-73.
doi:10.37040/geografie2021126010055
https://hdl.handle.net/21.15107/rcub_gery_1080 .

Stričević, Ljiljana S., Pavlović, Mila, Filipović, Ivan M., Radivojević, Aleksandar, Martić-Bursać, Nataša, Gocić, Milena, "Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018" in Geografie, 126, no. 1 (2021):55-73,
https://doi.org/10.37040/geografie2021126010055 .,
https://hdl.handle.net/21.15107/rcub_gery_1080 .