TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša
AU  - Ćurčić, Nina
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1396
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media SA
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša and Ćurčić, Nina and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media SA",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S., Ćurčić, N., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution
Frontiers Media SA., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković S, Ćurčić N, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša, Ćurčić, Nina, Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities" in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Đukić, Nikola N.
AU  - Vasiljević, Božica
AU  - Milošević, Đurađ
AU  - Valjarević, Aleksandar
AU  - Jakšić, Tatjana
AU  - Vasić, Predrag S.
AU  - Štrbac, Snežana
PY  - 2020
UR  - https://gery.gef.bg.ac.rs/handle/123456789/1069
AB  - The study is focused on diatom communities as indicators of the water quality of aquatic ecosystems. The study included watercourses of the Timok River Basin in eastern Serbia. Sampling of algological material and physicochemical measurements were conducted at 30 locations in September 2016. Non-metric multidimensional scaling (NMDS) showed that conductivity had an effect on the spatial variability of the diatom communities. At locations under influence of acid mine drainage along the Borska river, its tributaries and the Timok river, the diversity of benthic diatoms decreased and the abundance of metal tolerant taxa Achnanthidium minutissimum, Nitzschia capitellata and Nitzschia palea increased. Our study revealed that the combined effect of different pollutants significantly impacted on diatom assemblages; diatoms are good bioindicators of multiple pressures; and diatom indices with different types of pollution, may show an unreliable picture of the actual state, therefore, biological and physico-chemical parameters should also be observed when interpreting the results of a solely diatom-based assessment of the ecological condition of freshwaters.
PB  - Publ House Bulgarian Acad Sci, Sofia
T2  - Comptes Rendus De L Academie Bulgare Des Sciences
T1  - A water quality assessment based on benthic diatoms of the Timok river basin (eastern Serbia) under multiple anthropogenic pressures
VL  - 73
IS  - 12
SP  - 1696
EP  - 1702
DO  - 10.7546/CRABS.2020.12.09
UR  - https://hdl.handle.net/21.15107/rcub_gery_1069
ER  - 

@article{
author = "Đukić, Nikola N. and Vasiljević, Božica and Milošević, Đurađ and Valjarević, Aleksandar and Jakšić, Tatjana and Vasić, Predrag S. and Štrbac, Snežana",
year = "2020",
abstract = "The study is focused on diatom communities as indicators of the water quality of aquatic ecosystems. The study included watercourses of the Timok River Basin in eastern Serbia. Sampling of algological material and physicochemical measurements were conducted at 30 locations in September 2016. Non-metric multidimensional scaling (NMDS) showed that conductivity had an effect on the spatial variability of the diatom communities. At locations under influence of acid mine drainage along the Borska river, its tributaries and the Timok river, the diversity of benthic diatoms decreased and the abundance of metal tolerant taxa Achnanthidium minutissimum, Nitzschia capitellata and Nitzschia palea increased. Our study revealed that the combined effect of different pollutants significantly impacted on diatom assemblages; diatoms are good bioindicators of multiple pressures; and diatom indices with different types of pollution, may show an unreliable picture of the actual state, therefore, biological and physico-chemical parameters should also be observed when interpreting the results of a solely diatom-based assessment of the ecological condition of freshwaters.",
publisher = "Publ House Bulgarian Acad Sci, Sofia",
journal = "Comptes Rendus De L Academie Bulgare Des Sciences",
title = "A water quality assessment based on benthic diatoms of the Timok river basin (eastern Serbia) under multiple anthropogenic pressures",
volume = "73",
number = "12",
pages = "1696-1702",
doi = "10.7546/CRABS.2020.12.09",
url = "https://hdl.handle.net/21.15107/rcub_gery_1069"
}

Đukić, N. N., Vasiljević, B., Milošević, Đ., Valjarević, A., Jakšić, T., Vasić, P. S.,& Štrbac, S.. (2020). A water quality assessment based on benthic diatoms of the Timok river basin (eastern Serbia) under multiple anthropogenic pressures. in Comptes Rendus De L Academie Bulgare Des Sciences
Publ House Bulgarian Acad Sci, Sofia., 73(12), 1696-1702.
https://doi.org/10.7546/CRABS.2020.12.09
https://hdl.handle.net/21.15107/rcub_gery_1069

Đukić NN, Vasiljević B, Milošević Đ, Valjarević A, Jakšić T, Vasić PS, Štrbac S. A water quality assessment based on benthic diatoms of the Timok river basin (eastern Serbia) under multiple anthropogenic pressures. in Comptes Rendus De L Academie Bulgare Des Sciences. 2020;73(12):1696-1702.
doi:10.7546/CRABS.2020.12.09
https://hdl.handle.net/21.15107/rcub_gery_1069 .

Đukić, Nikola N., Vasiljević, Božica, Milošević, Đurađ, Valjarević, Aleksandar, Jakšić, Tatjana, Vasić, Predrag S., Štrbac, Snežana, "A water quality assessment based on benthic diatoms of the Timok river basin (eastern Serbia) under multiple anthropogenic pressures" in Comptes Rendus De L Academie Bulgare Des Sciences, 73, no. 12 (2020):1696-1702,
https://doi.org/10.7546/CRABS.2020.12.09 .,
https://hdl.handle.net/21.15107/rcub_gery_1069 .