TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag
AU  - Milinčić, Katarina M.
AU  - Todosijević, Marina
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Ćurčić, Nina B.
AU  - Stevanović, Nataša
AU  - Mihajlović, Ljiljana
AU  - Vukoičić, Danijela Ž.
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1496
AB  - The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.
PB  - Frontiers Media SA
T2  - Frontiers in Ecology and Evolution
T1  - The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)
VL  - 11
SP  - 1120006
DO  - 10.3389/fevo.2023.1120006
ER  - 

@article{
author = "Vesović, Nikola and Nenadić, Marija and Vranić, Sofija and Vujisić, Ljubodrag and Milinčić, Katarina M. and Todosijević, Marina and Dimkić, Ivica and Janakiev, Tamara and Ćurčić, Nina B. and Stevanović, Nataša and Mihajlović, Ljiljana and Vukoičić, Danijela Ž. and Ćurčić, Srećko",
year = "2023",
abstract = "The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Ecology and Evolution",
title = "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)",
volume = "11",
pages = "1120006",
doi = "10.3389/fevo.2023.1120006"
}

Vesović, N., Nenadić, M., Vranić, S., Vujisić, L., Milinčić, K. M., Todosijević, M., Dimkić, I., Janakiev, T., Ćurčić, N. B., Stevanović, N., Mihajlović, L., Vukoičić, D. Ž.,& Ćurčić, S.. (2023). The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution
Frontiers Media SA., 11, 1120006.
https://doi.org/10.3389/fevo.2023.1120006

Vesović N, Nenadić M, Vranić S, Vujisić L, Milinčić KM, Todosijević M, Dimkić I, Janakiev T, Ćurčić NB, Stevanović N, Mihajlović L, Vukoičić DŽ, Ćurčić S. The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution. 2023;11:1120006.
doi:10.3389/fevo.2023.1120006 .

Vesović, Nikola, Nenadić, Marija, Vranić, Sofija, Vujisić, Ljubodrag, Milinčić, Katarina M., Todosijević, Marina, Dimkić, Ivica, Janakiev, Tamara, Ćurčić, Nina B., Stevanović, Nataša, Mihajlović, Ljiljana, Vukoičić, Danijela Ž., Ćurčić, Srećko, "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)" in Frontiers in Ecology and Evolution, 11 (2023):1120006,
https://doi.org/10.3389/fevo.2023.1120006 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša
AU  - Ćurčić, Nina
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1396
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media SA
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša and Ćurčić, Nina and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media SA",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S., Ćurčić, N., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution
Frontiers Media SA., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković S, Ćurčić N, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša, Ćurčić, Nina, Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities" in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Cvetković, Milena
AU  - Brankov, Jovana
AU  - Ćurčić, Nevena
AU  - Pavlović, Sanja
AU  - Dobričić, Milica
AU  - Tretiakova, Tatiana N.
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1523
AB  - This research deals with the evaluation of tourist attractions in selected protected areas (PAs) in Serbia for the purpose of developing ecotourism. Using the integral AHP-SWOT method, two authentic mountain areas were analyzed—Stara Planina Nature Park and Suva Planina Special Nature Reserve. The results confirmed the presence of numerous natural and, to a lesser extent, anthropogenic resources that represent the basis for the future ecotourism activities. However, significant weaknesses were also registered, which are reflected in overall poverty, underdeveloped infrastructure and depopulation. The analysis also showed that the highest rated factors for the
development of ecotourism are local products that should be offered on the regional market, as well as the use of the geographical position of both areas (near the state border) through the use of donor funds (IPA funds). The application of the AHP-SWOT method enabled the definition and prioritization of several development strategies, of which the ones with the greatest weight are based on the use of the variegated biodiversity of both PAs. This research advances knowledge about strategic decision-making in PAs and could be beneficial to destination managers for the creation of successful strategic plans. Results suggest that future actions should be aimed at maximizing the detected advantages, which means creating an appropriate development policy, drafting legislation, encouraging social mobilization activities and determining the institutional elements for the development and management of ecotourism.
PB  - Basel : MDPI
T2  - Sustainability
T1  - Protected Natural Areas and Ecotourism - Priority Strategies for Future Development in Selected Serbian Case Studies
VL  - 15
IS  - 21
SP  - 15621
DO  - 10.3390/su152115621
ER  - 

@article{
author = "Cvetković, Milena and Brankov, Jovana and Ćurčić, Nevena and Pavlović, Sanja and Dobričić, Milica and Tretiakova, Tatiana N.",
year = "2023",
abstract = "This research deals with the evaluation of tourist attractions in selected protected areas (PAs) in Serbia for the purpose of developing ecotourism. Using the integral AHP-SWOT method, two authentic mountain areas were analyzed—Stara Planina Nature Park and Suva Planina Special Nature Reserve. The results confirmed the presence of numerous natural and, to a lesser extent, anthropogenic resources that represent the basis for the future ecotourism activities. However, significant weaknesses were also registered, which are reflected in overall poverty, underdeveloped infrastructure and depopulation. The analysis also showed that the highest rated factors for the
development of ecotourism are local products that should be offered on the regional market, as well as the use of the geographical position of both areas (near the state border) through the use of donor funds (IPA funds). The application of the AHP-SWOT method enabled the definition and prioritization of several development strategies, of which the ones with the greatest weight are based on the use of the variegated biodiversity of both PAs. This research advances knowledge about strategic decision-making in PAs and could be beneficial to destination managers for the creation of successful strategic plans. Results suggest that future actions should be aimed at maximizing the detected advantages, which means creating an appropriate development policy, drafting legislation, encouraging social mobilization activities and determining the institutional elements for the development and management of ecotourism.",
publisher = "Basel : MDPI",
journal = "Sustainability",
title = "Protected Natural Areas and Ecotourism - Priority Strategies for Future Development in Selected Serbian Case Studies",
volume = "15",
number = "21",
pages = "15621",
doi = "10.3390/su152115621"
}

Cvetković, M., Brankov, J., Ćurčić, N., Pavlović, S., Dobričić, M.,& Tretiakova, T. N.. (2023). Protected Natural Areas and Ecotourism - Priority Strategies for Future Development in Selected Serbian Case Studies. in Sustainability
Basel : MDPI., 15(21), 15621.
https://doi.org/10.3390/su152115621

Cvetković M, Brankov J, Ćurčić N, Pavlović S, Dobričić M, Tretiakova TN. Protected Natural Areas and Ecotourism - Priority Strategies for Future Development in Selected Serbian Case Studies. in Sustainability. 2023;15(21):15621.
doi:10.3390/su152115621 .

Cvetković, Milena, Brankov, Jovana, Ćurčić, Nevena, Pavlović, Sanja, Dobričić, Milica, Tretiakova, Tatiana N., "Protected Natural Areas and Ecotourism - Priority Strategies for Future Development in Selected Serbian Case Studies" in Sustainability, 15, no. 21 (2023):15621,
https://doi.org/10.3390/su152115621 . .

TY  - JOUR
AU  - Lukić, Vesna
AU  - Lović Obradović, Suzana
AU  - Stojilković Gnjatović, Jelena
PY  - 2023
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1723
UR  - http://iriss.idn.org.rs/1436/
AB  - Recent empirical research on internal migration has focused on the change in migration volume, before and after the outbreak of the COVID-19 pandemic, by the degree of urbanization. Less is known about the types of rural areas that are attracting internal migrants. This study aims to explore the changes in internal rural migration patterns in Serbia due to the COVID-19 pandemic, focusing on the types of rural settlements as places of destinations and types of urban settlements as places of the origin of migrants. Relying on the additionally processed official national annual statistical data for the period 2018–2021, we offer findings on the differences in volume and socio-demographic characteristics of migrants across six internal migration flows (from small towns, medium-sized towns, and large cities, to suburban and non-suburban rural settlements). The results reveal that the pandemic affected an increase of rural–rural migration as well as rural in-migration from mediumsized towns. We also find that after an initial drop in values for in- and out-migration rates in 2020, relocation intensity exceeded the pre-pandemic level in 2021, with a positive net migration rate for all age groups except 15–30. The unfavorable economic characteristics of rural in-migrants shifted slightly in pandemic years since the share of active and population with personal means rose in non-suburban settlements, while the percentage of dependent population decreased. The opposite holds for suburban settlements.
PB  - Bonn : University of Bonn
T2  - Erdkunde
T1  - Did the Covid-19 Pandemic Change Internal Rural Migration Patterns in Serbia?
VL  - 77
IS  - 3
SP  - 233
EP  - 249
DO  - 10.3112/erdkunde.2023.03.04
ER  - 

@article{
author = "Lukić, Vesna and Lović Obradović, Suzana and Stojilković Gnjatović, Jelena",
year = "2023",
abstract = "Recent empirical research on internal migration has focused on the change in migration volume, before and after the outbreak of the COVID-19 pandemic, by the degree of urbanization. Less is known about the types of rural areas that are attracting internal migrants. This study aims to explore the changes in internal rural migration patterns in Serbia due to the COVID-19 pandemic, focusing on the types of rural settlements as places of destinations and types of urban settlements as places of the origin of migrants. Relying on the additionally processed official national annual statistical data for the period 2018–2021, we offer findings on the differences in volume and socio-demographic characteristics of migrants across six internal migration flows (from small towns, medium-sized towns, and large cities, to suburban and non-suburban rural settlements). The results reveal that the pandemic affected an increase of rural–rural migration as well as rural in-migration from mediumsized towns. We also find that after an initial drop in values for in- and out-migration rates in 2020, relocation intensity exceeded the pre-pandemic level in 2021, with a positive net migration rate for all age groups except 15–30. The unfavorable economic characteristics of rural in-migrants shifted slightly in pandemic years since the share of active and population with personal means rose in non-suburban settlements, while the percentage of dependent population decreased. The opposite holds for suburban settlements.",
publisher = "Bonn : University of Bonn",
journal = "Erdkunde",
title = "Did the Covid-19 Pandemic Change Internal Rural Migration Patterns in Serbia?",
volume = "77",
number = "3",
pages = "233-249",
doi = "10.3112/erdkunde.2023.03.04"
}

Lukić, V., Lović Obradović, S.,& Stojilković Gnjatović, J.. (2023). Did the Covid-19 Pandemic Change Internal Rural Migration Patterns in Serbia?. in Erdkunde
Bonn : University of Bonn., 77(3), 233-249.
https://doi.org/10.3112/erdkunde.2023.03.04

Lukić V, Lović Obradović S, Stojilković Gnjatović J. Did the Covid-19 Pandemic Change Internal Rural Migration Patterns in Serbia?. in Erdkunde. 2023;77(3):233-249.
doi:10.3112/erdkunde.2023.03.04 .

Lukić, Vesna, Lović Obradović, Suzana, Stojilković Gnjatović, Jelena, "Did the Covid-19 Pandemic Change Internal Rural Migration Patterns in Serbia?" in Erdkunde, 77, no. 3 (2023):233-249,
https://doi.org/10.3112/erdkunde.2023.03.04 . .