TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Popović, Vladan
AU  - Nevenić, Marija
AU  - Ristić, Danijela
AU  - Rakonjac, Ljubinko
AU  - Ćirković-Mitrović, Tatjana
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://gery.gef.bg.ac.rs/handle/123456789/1547
AB  - This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).
PB  - Belgrade : Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers
VL  - 66
IS  - 4
SP  - 1485
EP  - 1492
DO  - 10.2298/ABS1404485L
ER  - 

@article{
author = "Lučić, Aleksandar and Popović, Vladan and Nevenić, Marija and Ristić, Danijela and Rakonjac, Ljubinko and Ćirković-Mitrović, Tatjana and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).",
publisher = "Belgrade : Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers",
volume = "66",
number = "4",
pages = "1485-1492",
doi = "10.2298/ABS1404485L"
}

Lučić, A., Popović, V., Nevenić, M., Ristić, D., Rakonjac, L., Ćirković-Mitrović, T.,& Mladenović-Drinić, S.. (2014). Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers. in Archives of Biological Sciences
Belgrade : Serbian Biological Society., 66(4), 1485-1492.
https://doi.org/10.2298/ABS1404485L

Lučić A, Popović V, Nevenić M, Ristić D, Rakonjac L, Ćirković-Mitrović T, Mladenović-Drinić S. Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers. in Archives of Biological Sciences. 2014;66(4):1485-1492.
doi:10.2298/ABS1404485L .

Lučić, Aleksandar, Popović, Vladan, Nevenić, Marija, Ristić, Danijela, Rakonjac, Ljubinko, Ćirković-Mitrović, Tatjana, Mladenović-Drinić, Snežana, "Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers" in Archives of Biological Sciences, 66, no. 4 (2014):1485-1492,
https://doi.org/10.2298/ABS1404485L . .